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mouse monoclonal anti nucleolin  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse monoclonal anti nucleolin
    Mouse Monoclonal Anti Nucleolin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+nucleolin/C23+Antibody/pmc12799909-4-0-7
    Average 95 stars, based on 460 article reviews
    mouse monoclonal anti nucleolin - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Recombinant:

    Article Title: Cohesin regulates alternative splicing
    Article Snippet: The primary antibodies used in and fig. S11A were as follows: mouse monoclonal anti-BRD4 (Sigma-Aldrich, AMAB90841), rabbit polyclonal anti-RAD21 (Abcam, ab154769), rabbit polyclonal anti-SMC3 (Abcam, ab9263), and anti-Tbp1 (Abcam, ab63766).

    Article Title: Mechanical control of the alternative splicing factor PTBP1 regulates extracellular matrix stiffness induced proliferation and cell spreading
    Article Snippet: Mouse monoclonal anti-nucleolin , Santa Cruz , #sc-8031; RRID: AB_670271.

    Article Title: Protocol to identify mechanosensitive nuclear proteins using tunable actomyosin contractility and proximity biotinylation in mammalian cells
    Article Snippet: Mouse monoclonal anti-nucleolin, antibody dilution: 1/300 , Santa Cruz , #sc-8031; RRID: AB_670271.

    Selection:

    Article Title: Cohesin regulates alternative splicing
    Article Snippet: The primary antibodies used in and fig. S11A were as follows: mouse monoclonal anti-BRD4 (Sigma-Aldrich, AMAB90841), rabbit polyclonal anti-RAD21 (Abcam, ab154769), rabbit polyclonal anti-SMC3 (Abcam, ab9263), and anti-Tbp1 (Abcam, ab63766).

    Article Title: Mechanical control of the alternative splicing factor PTBP1 regulates extracellular matrix stiffness induced proliferation and cell spreading
    Article Snippet: Mouse monoclonal anti-nucleolin , Santa Cruz , #sc-8031; RRID: AB_670271.

    Article Title: Protocol to identify mechanosensitive nuclear proteins using tunable actomyosin contractility and proximity biotinylation in mammalian cells
    Article Snippet: Mouse monoclonal anti-nucleolin, antibody dilution: 1/300 , Santa Cruz , #sc-8031; RRID: AB_670271.

    Staining:

    Article Title: Cohesin regulates alternative splicing
    Article Snippet: The primary antibodies used in and fig. S11A were as follows: mouse monoclonal anti-BRD4 (Sigma-Aldrich, AMAB90841), rabbit polyclonal anti-RAD21 (Abcam, ab154769), rabbit polyclonal anti-SMC3 (Abcam, ab9263), and anti-Tbp1 (Abcam, ab63766).

    Article Title: Mechanical control of the alternative splicing factor PTBP1 regulates extracellular matrix stiffness induced proliferation and cell spreading
    Article Snippet: Mouse monoclonal anti-nucleolin , Santa Cruz , #sc-8031; RRID: AB_670271.

    Article Title: Protocol to identify mechanosensitive nuclear proteins using tunable actomyosin contractility and proximity biotinylation in mammalian cells
    Article Snippet: Mouse monoclonal anti-nucleolin, antibody dilution: 1/300 , Santa Cruz , #sc-8031; RRID: AB_670271.

    Negative Control:

    Article Title: Cohesin regulates alternative splicing
    Article Snippet: The primary antibodies used in and fig. S11A were as follows: mouse monoclonal anti-BRD4 (Sigma-Aldrich, AMAB90841), rabbit polyclonal anti-RAD21 (Abcam, ab154769), rabbit polyclonal anti-SMC3 (Abcam, ab9263), and anti-Tbp1 (Abcam, ab63766).

    Article Title: Mechanical control of the alternative splicing factor PTBP1 regulates extracellular matrix stiffness induced proliferation and cell spreading
    Article Snippet: Mouse monoclonal anti-nucleolin , Santa Cruz , #sc-8031; RRID: AB_670271.

    Article Title: Protocol to identify mechanosensitive nuclear proteins using tunable actomyosin contractility and proximity biotinylation in mammalian cells
    Article Snippet: Mouse monoclonal anti-nucleolin, antibody dilution: 1/300 , Santa Cruz , #sc-8031; RRID: AB_670271.

    Software:

    Article Title: Cohesin regulates alternative splicing
    Article Snippet: The primary antibodies used in and fig. S11A were as follows: mouse monoclonal anti-BRD4 (Sigma-Aldrich, AMAB90841), rabbit polyclonal anti-RAD21 (Abcam, ab154769), rabbit polyclonal anti-SMC3 (Abcam, ab9263), and anti-Tbp1 (Abcam, ab63766).

    Article Title: Mechanical control of the alternative splicing factor PTBP1 regulates extracellular matrix stiffness induced proliferation and cell spreading
    Article Snippet: Mouse monoclonal anti-nucleolin , Santa Cruz , #sc-8031; RRID: AB_670271.

    Article Title: Protocol to identify mechanosensitive nuclear proteins using tunable actomyosin contractility and proximity biotinylation in mammalian cells
    Article Snippet: Mouse monoclonal anti-nucleolin, antibody dilution: 1/300 , Santa Cruz , #sc-8031; RRID: AB_670271.

    Binding Assay:

    Article Title: Cohesin regulates alternative splicing
    Article Snippet: The primary antibodies used in and fig. S11A were as follows: mouse monoclonal anti-BRD4 (Sigma-Aldrich, AMAB90841), rabbit polyclonal anti-RAD21 (Abcam, ab154769), rabbit polyclonal anti-SMC3 (Abcam, ab9263), and anti-Tbp1 (Abcam, ab63766).

    Article Title: Mechanical control of the alternative splicing factor PTBP1 regulates extracellular matrix stiffness induced proliferation and cell spreading
    Article Snippet: Mouse monoclonal anti-nucleolin , Santa Cruz , #sc-8031; RRID: AB_670271.

    Article Title: Protocol to identify mechanosensitive nuclear proteins using tunable actomyosin contractility and proximity biotinylation in mammalian cells
    Article Snippet: Mouse monoclonal anti-nucleolin, antibody dilution: 1/300 , Santa Cruz , #sc-8031; RRID: AB_670271.

    Immunoprecipitation:

    Article Title: Cohesin regulates alternative splicing
    Article Snippet: The primary antibodies used in and fig. S11A were as follows: mouse monoclonal anti-BRD4 (Sigma-Aldrich, AMAB90841), rabbit polyclonal anti-RAD21 (Abcam, ab154769), rabbit polyclonal anti-SMC3 (Abcam, ab9263), and anti-Tbp1 (Abcam, ab63766).

    Article Title: Mechanical control of the alternative splicing factor PTBP1 regulates extracellular matrix stiffness induced proliferation and cell spreading
    Article Snippet: Mouse monoclonal anti-nucleolin , Santa Cruz , #sc-8031; RRID: AB_670271.

    Article Title: Protocol to identify mechanosensitive nuclear proteins using tunable actomyosin contractility and proximity biotinylation in mammalian cells
    Article Snippet: Mouse monoclonal anti-nucleolin, antibody dilution: 1/300 , Santa Cruz , #sc-8031; RRID: AB_670271.

    Western Blot:

    Article Title: Cohesin regulates alternative splicing
    Article Snippet: The primary antibodies used in and fig. S11A were as follows: mouse monoclonal anti-BRD4 (Sigma-Aldrich, AMAB90841), rabbit polyclonal anti-RAD21 (Abcam, ab154769), rabbit polyclonal anti-SMC3 (Abcam, ab9263), and anti-Tbp1 (Abcam, ab63766).

    Article Title: Mechanical control of the alternative splicing factor PTBP1 regulates extracellular matrix stiffness induced proliferation and cell spreading
    Article Snippet: Mouse monoclonal anti-nucleolin , Santa Cruz , #sc-8031; RRID: AB_670271.

    Article Title: Protocol to identify mechanosensitive nuclear proteins using tunable actomyosin contractility and proximity biotinylation in mammalian cells
    Article Snippet: Mouse monoclonal anti-nucleolin, antibody dilution: 1/300 , Santa Cruz , #sc-8031; RRID: AB_670271.

    Incubation:

    Article Title: Cohesin regulates alternative splicing
    Article Snippet: The primary antibodies used in and fig. S11A were as follows: mouse monoclonal anti-BRD4 (Sigma-Aldrich, AMAB90841), rabbit polyclonal anti-RAD21 (Abcam, ab154769), rabbit polyclonal anti-SMC3 (Abcam, ab9263), and anti-Tbp1 (Abcam, ab63766).

    Article Title: Mechanical control of the alternative splicing factor PTBP1 regulates extracellular matrix stiffness induced proliferation and cell spreading
    Article Snippet: Mouse monoclonal anti-nucleolin , Santa Cruz , #sc-8031; RRID: AB_670271.

    Article Title: Protocol to identify mechanosensitive nuclear proteins using tunable actomyosin contractility and proximity biotinylation in mammalian cells
    Article Snippet: Mouse monoclonal anti-nucleolin, antibody dilution: 1/300 , Santa Cruz , #sc-8031; RRID: AB_670271.

    Pull Down Assay:

    Article Title: Cohesin regulates alternative splicing
    Article Snippet: The primary antibodies used in and fig. S11A were as follows: mouse monoclonal anti-BRD4 (Sigma-Aldrich, AMAB90841), rabbit polyclonal anti-RAD21 (Abcam, ab154769), rabbit polyclonal anti-SMC3 (Abcam, ab9263), and anti-Tbp1 (Abcam, ab63766).

    Article Title: Mechanical control of the alternative splicing factor PTBP1 regulates extracellular matrix stiffness induced proliferation and cell spreading
    Article Snippet: Mouse monoclonal anti-nucleolin , Santa Cruz , #sc-8031; RRID: AB_670271.

    Article Title: Protocol to identify mechanosensitive nuclear proteins using tunable actomyosin contractility and proximity biotinylation in mammalian cells
    Article Snippet: Mouse monoclonal anti-nucleolin, antibody dilution: 1/300 , Santa Cruz , #sc-8031; RRID: AB_670271.



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    Santa Cruz Biotechnology anti nucleolin c23 ms 3 mouse monoclonal antibody
    Figure 10. Dual RNAscope and immunostaining in WT1 and DMD immortalized myoblasts. (A) 37–42 and 63–75 RNAscope probes were used in combination with a <t>monoclonal</t> antibody against the human <t>nucleolin</t> protein. Each single DMD transcript is represented as a distinct red dot, whereas nucleolin protein is represented as a green signal. Counterstain: Gill’s Hematoxylin. (B) Colocalization of dystrophin transcript and nucleolin protein in immortalized myoblasts. Using the 37–42 probe, we found about 45% in WT1 and a mean of 42% in DMD of Dp427 overlapping with nucleolin signals. No statistically significant differences were observed between samples. Using probe 63–75, we showed lower dystrophin and nucleolin colocalization in both WT1 (26%) and DMD (mean of 34%). No statistically significant differences were found for 63–75 signal between WT1 and DMD. Bar: SEM. **p < 0.001; *p < 0.05.
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    Image Search Results


    Top 25 most abundant AFAP-interacting proteins.

    Journal: Frontiers in Microbiology

    Article Title: Anaplasma phagocytophilum AFAP targets the host nucleolus and inhibits induced apoptosis

    doi: 10.3389/fmicb.2024.1533640

    Figure Lengend Snippet: Top 25 most abundant AFAP-interacting proteins.

    Article Snippet: Permeabilized cells were incubated with a mouse monoclonal anti-nucleolin antibody (cat#:sc-17826, Santa Cruz Biotechnology) or mouse monoclonal anti-NPM1 antibody (cat#: sc-32256, Santa Cruz Biotechnology) at the dilution of 1:100 for 1 h at 37°C before incubation with Alexa Fluor 555-conjugated goat anti-mouse IgG antibody (1:200 dilution, Molecular Probes, Invitrogen).

    Techniques:

    Colocalization assay for AFAP and nucleolin, and AFAP and NPM1. RF/6A cells were transfected for 1 day with a plasmid expressing AFAP-GFP (A,B) or GFP (C,D) , followed by incubation with mouse monoclonal anti-nucleolin (A,C) or anti-nucleophosmin (B,D) antibody and Alexa Fluor 555-conjugated goat anti-mouse IgG antibody. DAPI was used to stain the nucleus. Scale bars: 10 μm.

    Journal: Frontiers in Microbiology

    Article Title: Anaplasma phagocytophilum AFAP targets the host nucleolus and inhibits induced apoptosis

    doi: 10.3389/fmicb.2024.1533640

    Figure Lengend Snippet: Colocalization assay for AFAP and nucleolin, and AFAP and NPM1. RF/6A cells were transfected for 1 day with a plasmid expressing AFAP-GFP (A,B) or GFP (C,D) , followed by incubation with mouse monoclonal anti-nucleolin (A,C) or anti-nucleophosmin (B,D) antibody and Alexa Fluor 555-conjugated goat anti-mouse IgG antibody. DAPI was used to stain the nucleus. Scale bars: 10 μm.

    Article Snippet: Permeabilized cells were incubated with a mouse monoclonal anti-nucleolin antibody (cat#:sc-17826, Santa Cruz Biotechnology) or mouse monoclonal anti-NPM1 antibody (cat#: sc-32256, Santa Cruz Biotechnology) at the dilution of 1:100 for 1 h at 37°C before incubation with Alexa Fluor 555-conjugated goat anti-mouse IgG antibody (1:200 dilution, Molecular Probes, Invitrogen).

    Techniques: Transfection, Plasmid Preparation, Expressing, Incubation, Staining

    Figure 10. Dual RNAscope and immunostaining in WT1 and DMD immortalized myoblasts. (A) 37–42 and 63–75 RNAscope probes were used in combination with a monoclonal antibody against the human nucleolin protein. Each single DMD transcript is represented as a distinct red dot, whereas nucleolin protein is represented as a green signal. Counterstain: Gill’s Hematoxylin. (B) Colocalization of dystrophin transcript and nucleolin protein in immortalized myoblasts. Using the 37–42 probe, we found about 45% in WT1 and a mean of 42% in DMD of Dp427 overlapping with nucleolin signals. No statistically significant differences were observed between samples. Using probe 63–75, we showed lower dystrophin and nucleolin colocalization in both WT1 (26%) and DMD (mean of 34%). No statistically significant differences were found for 63–75 signal between WT1 and DMD. Bar: SEM. **p < 0.001; *p < 0.05.

    Journal: Scientific reports

    Article Title: mRNA in situ hybridization exhibits unbalanced nuclear/cytoplasmic dystrophin transcript repartition in Duchenne myogenic cells and skeletal muscle biopsies.

    doi: 10.1038/s41598-023-43134-6

    Figure Lengend Snippet: Figure 10. Dual RNAscope and immunostaining in WT1 and DMD immortalized myoblasts. (A) 37–42 and 63–75 RNAscope probes were used in combination with a monoclonal antibody against the human nucleolin protein. Each single DMD transcript is represented as a distinct red dot, whereas nucleolin protein is represented as a green signal. Counterstain: Gill’s Hematoxylin. (B) Colocalization of dystrophin transcript and nucleolin protein in immortalized myoblasts. Using the 37–42 probe, we found about 45% in WT1 and a mean of 42% in DMD of Dp427 overlapping with nucleolin signals. No statistically significant differences were observed between samples. Using probe 63–75, we showed lower dystrophin and nucleolin colocalization in both WT1 (26%) and DMD (mean of 34%). No statistically significant differences were found for 63–75 signal between WT1 and DMD. Bar: SEM. **p < 0.001; *p < 0.05.

    Article Snippet: Nucleolin protein was immunoprecipitated with anti-nucleolin C23 MS-3 mouse monoclonal antibody (5 μg; Santa Cruz) using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore).

    Techniques: RNAscope, Immunostaining